me180 cervical cancer cell line  (ATCC)

Journal: Nature Communications

Article Title: Pretreatment tumor-related leukocytosis misleads positron emission tomography-computed tomography during lymph node staging in gynecological malignancies

doi: 10.1038/s41467-020-15186-z

Figure Lengend Snippet: a Representative image of G-CSF mRNA expression in ME180-Control or ME180-GCSF cells as evaluated by RT-PCR. b Representative G-CSF staining in ME180-Control cells or ME180-GCSF cells-derived tumors. c WBC/granulocyte counts of ME180-Control-derived tumor-bearing rats and ME180-GCSF-derived tumor-bearing rats (three rats per group). Rats were subcutaneously inoculated with ME180-Control or ME180-GCSF cells. Three weeks after inoculation, their subcutaneous tumors or peripheral blood samples were collected for analyses. d 18F-FDG-PET/CT scan of ME180-Control-derived tumor- and ME180-GCSF-derived tumor-bearing rats. Rats were subcutaneously inoculated with ME180-Control or ME180-GCSF cells. Three weeks after inoculation, 18F-FDG-PET/CT was performed. ME180-GCSF-derived tumor-bearing rats showed significant 18F-FDG-uptake in the PALN (circle). e PALNs resected after 18F-FDG-PET/CT. f Representative pathological findings from the PALNs resected after 18F-FDG-PET/CT (hematoxylin and eosin). Both of the images contain no malignant cells. g Effects of tumor-derived G-CSF on the induction of MDSC in rat models of cervical cancer. CD11b/c + HIS48 + cell populations detected in the peripheral blood and lymph nodes. Rats were subcutaneously inoculated with ME180-Control or ME180-GCSF cells. Three weeks after inoculation, the number of MDSC was evaluated by flow cytometry (three rats per group). h Effects of tumor-derived G-CSF and anti-Gr-1 antibody on PALN in mice models of cervical cancer. i Effects of anti-Gr-1 antibody on MDSC induction in mice models of cervical cancer. CD11b + Gr1 + cell populations detected in the peripheral blood and lymph nodes. h , i ME180-Control- or ME180-GCSF-derived tumor-bearing mice were treated with either control IgG or anti-Gr-1 antibody for three weeks (five mice per group). Then, the number of MDSC was evaluated by flow cytometry (five mice per group). j Ability of CD11b + Gr-1 + cells to suppress CD8 + T cell assessed by T-cell proliferation assay. CD11b + Gr-1 + cells (MDSC) were isolated from spleen of ME180-GCSF-derived tumor-bearing mouse. CD8 + T cells (2 × 10 5 cells/well) were isolated from spleen of Balb/c mice and co-cultured with MDSC at indicated ratios. Cells were incubated for 72 h, after which BrdU was added for an additional 24 h. T cell proliferation was determined by BrdU incorporation ( n = 6). Error bars indicate mean ± SD. Statistical significance was assessed using two-sided Welch t test. bp, base pairs. Scale bar, 50 μm. Source data are provided as a Source Data file.

Article Snippet: ME180 human cervical cancer cell line and Ishikawa human endometrial cancer cell line were purchased from the American Type Culture Collection.

Techniques: Expressing, Control, Reverse Transcription Polymerase Chain Reaction, Staining, Derivative Assay, Positron Emission Tomography-Computed Tomography, Flow Cytometry, Proliferation Assay, Isolation, Cell Culture, Incubation, BrdU Incorporation Assay

Journal: Nature Communications

Article Title: Pretreatment tumor-related leukocytosis misleads positron emission tomography-computed tomography during lymph node staging in gynecological malignancies

doi: 10.1038/s41467-020-15186-z

Figure Lengend Snippet: a The SUV max of on the site of S100A8/A9 heterodimer injection by 18F-FDG-PET/CT. Mice were subcutaneously injected with indicated concentrations of S100A8/A9 heterodimer. 18F-FDG-PET/CT was performed 24 h after the injection, and the SUV max of the injection site was analyzed (PBS, n = 3; others, n = 2). b Representative immunoreactivities of resected paraaortic lymph nodes for S100A8 and S100A9 (ME180-Control-derived tumor- or ME180-GCSF-derived tumor-bearing rats). Scale bars, 50 μm. c Representative image of S100a8 and S100a9 mRNA expression in MDSC of ME180-GCSF-derived tumor-bearing mice as evaluated by RT-PCR. bp, base pairs. Source data are provided as a Source Data file.

Article Snippet: ME180 human cervical cancer cell line and Ishikawa human endometrial cancer cell line were purchased from the American Type Culture Collection.

Techniques: Injection, Positron Emission Tomography-Computed Tomography, Control, Derivative Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: GPX2 suppression of H 2 O 2 stress regulates cervical cancer metastasis and apoptosis via activation of the β-catenin-WNT pathway

doi: 10.2147/OTT.S208781

Figure Lengend Snippet: Expression of GPX2 in cervical clinical specimens and cell lines. Notes: ( A ) GPX2 expression in cervical squamous cell carcinoma (CESE) clinical specimens obtained from the TCGA database. ( B ) GPX2 expression in cervical carcinoma cell lines ME180 and HeLa. ( C ) Western blot assay and qRT-PCR assay verify the transfection effect. Means ± S.D. for three independent experiments are shown. ** P <0.01; *** P <0.001; ns, not significant.

Article Snippet: Human cervical cancer cell lines ME180 and HeLa were purchased from the American Type Culture Collection.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Transfection

Journal: OncoTargets and therapy

Article Title: GPX2 suppression of H 2 O 2 stress regulates cervical cancer metastasis and apoptosis via activation of the β-catenin-WNT pathway

doi: 10.2147/OTT.S208781

Figure Lengend Snippet: GPX2 promotes the migration and invasion of ME180 and HeLa cells. Notes: ( A, B ) The cellular migration and invasion potential of ME180 and HeLa were assessed using transwell assay, cell numbers on the underside of the filter was calculated as the total count from 10 random fields per filter. Means ± S.D. for three independent experiments are shown. ** P <0.01; *** P <0.001.

Article Snippet: Human cervical cancer cell lines ME180 and HeLa were purchased from the American Type Culture Collection.

Techniques: Migration, Transwell Assay

Journal: OncoTargets and therapy

Article Title: GPX2 suppression of H 2 O 2 stress regulates cervical cancer metastasis and apoptosis via activation of the β-catenin-WNT pathway

doi: 10.2147/OTT.S208781

Figure Lengend Snippet: GPX2 affects the expression of EMT markers in cervical cancer cell lines. Notes: ( A, B ) qRT-PCR and Western blot for E-cadherin and Vimentin in ME180 and HeLa cells after transfection. Means ± S.D. for three independent experiments are shown. *** P <0.001.

Article Snippet: Human cervical cancer cell lines ME180 and HeLa were purchased from the American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection

Journal: OncoTargets and therapy

Article Title: GPX2 suppression of H 2 O 2 stress regulates cervical cancer metastasis and apoptosis via activation of the β-catenin-WNT pathway

doi: 10.2147/OTT.S208781

Figure Lengend Snippet: Identification of GPX2 as a key regulator of intracellular H 2 O 2 levels and an inhibitor of apoptosis. Notes: ( A ) FACS analysis of ROS levels using DCF-DA probe in ME180 and HeLa cell lines after transfection. ( B ) Colony formation assay of ME180 and HeLa cells after transfection in the presence or absence of 0.2 mmol/L NAC and 400 µmol/L H 2 O 2 ; the sum of the clone area was calculated by the Image-Pro Plus 6.0. ( C ) Western blot analysis of the activated caspase-3 protein levels in ME180 and HeLa cells after transfection. Means ± S.D. for three independent experiments are shown. * P <0.05; ** P <0.01; *** P <0.001; ns, not significant.

Article Snippet: Human cervical cancer cell lines ME180 and HeLa were purchased from the American Type Culture Collection.

Techniques: Transfection, Colony Assay, Western Blot

Journal: Cancer Cell International

Article Title: Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells

doi: 10.1186/s12935-016-0379-1

Figure Lengend Snippet: Forced expression of TFF3 in cervical cancer cells modulates the expression of malignant progression-related gene markers. a Determination of endogenous expression of TFF3 protein by Western blot in the cervical cancer cells lines CaSki, SiHa, Me180 and Hela and human non-tumor keratinocyte line HaCaT. b Semi-quantitative RT-PCR analysis was used to assess the mRNA levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3 as described in “ ” section. c Western blot analysis was used to assess the protein levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3. d , e Quantitative PCR analysis quantifying the change in expression of various genes associated with malignant progression in SiHa-TFF3/Hela cells. Change in gene expression is expressed as fold difference, respectively. Fold change values are representative of three independent experiments

Article Snippet: Human cervical cancer cell lines SiHa, CaSki, Hela, Me180 and human non-tumor keratinocyte line HaCaT were obtained from Nanjing KeyGen Biotech Co, Ltd (Nanjing,China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Gene Expression